کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767321 | 1050718 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
(-)-Epicatechin elevates nitric oxide in endothelial cells via inhibition of NADPH oxidase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Dietary (-)-epicatechin is known to improve bioactivity of NO in arterial endothelium of humans, but the mode of action is unclear. We used the fluorophore 4,5-diaminofluorescein diacetate to visualize the NO level in living human umbilical vein endothelial cells (HUVEC). Untreated cells showed only a weak signal, whereas pretreatment with (-)-epicatechin (10 μM) or apocynin (100 μM) elevated the NO level. The effects were more pronounced when the cells were treated with angiotensin II with or without preloading of the cells with NO via PAPA-NONOate. While (-)-epicatechin scavenged O2·-, its O-methylated metabolites prevented O2·- generation through inhibition of endothelial NADPH oxidase activity, even more strongly than apocynin. From the effect of 3,5-dinitrocatechol, an inhibitor of catechol-O-methyltransferase (COMT), on HUVEC it is concluded that (-)-epicatechin serves as 'prodrug' for conversion to apocynin-like NADPH oxidase inhibitors. These data indicate an NO-preserving effect of (-)-epicatechin via suppression of O2·--mediated loss of NO.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 359, Issue 3, 3 August 2007, Pages 828-833
Journal: Biochemical and Biophysical Research Communications - Volume 359, Issue 3, 3 August 2007, Pages 828-833
نویسندگان
Yvonne Steffen, Tankred Schewe, Helmut Sies,