کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767419 | 1050733 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The diffusive search mechanism of processive myosin class-V motor involves directional steps along actin subunits
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
It is widely accepted that the vesicle-transporter myosin-V moves processively along F-actin with large steps of â¼36Â nm using a hand-over-hand mechanism. A key question is how does the rear head of two-headed myosin-V search for the forward actin target in the forward direction. Scanning probe nanometry was used to resolve this underlying search process, which was made possible by attaching the head to a relatively large probe. One-headed myosin-V undergoes directional diffusion with â¼5.5Â nm substeps to develop an average displacement of â¼20Â nm, which was independent of the neck length (2IQ and 6IQ motifs). Two-headed myosin-V showed several â¼5.5Â nm substeps within each processive â¼36Â nm step. These results suggest that the myosin-V head searches in the forward direction for the actin target using directional diffusion on the actin subunits according to a potential slope created along the actin helix.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 2, 9 March 2007, Pages 379-384
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 2, 9 March 2007, Pages 379-384
نویسندگان
Takuya Okada, Hiroto Tanaka, Atsuko Hikikoshi Iwane, Kazuo Kitamura, Mitsuo Ikebe, Toshio Yanagida,