کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10767452 1050733 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Upstream stimulatory factors regulate the C/EBPα gene during differentiation of 3T3-L1 preadipocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Upstream stimulatory factors regulate the C/EBPα gene during differentiation of 3T3-L1 preadipocytes
چکیده انگلیسی
During adipocyte differentiation, CCAAT/enhancer-binding protein α (C/EBPα) functions as a pleiotropic transcriptional activator of numerous adipocyte genes. The promoter of the C/EBPα gene has an E-box upstream of C/EBP binding site. Deletion or mutation of the E-box decreases promoter activity, suggesting that the E-box participates in the regulation of C/EBPα expression. Protein binding to the E-box during the adipocyte differentiation is increased as indicated by EMSA and UV cross-linking. Purification of the E-box binding proteins from differentiated 3T3-L1 adipocytes, showed that USF and AP-4 are associated with the E-box. Supershift analysis showed that USF1 and USF2 bind to this element as heterodimers, whereas the addition of anti-AP-4 antibody enhanced the binding complex, suggesting that AP-4 negatively regulates the promoter activity. The expression of AP-4 is reciprocally regulated with USF-1 during adipocyte differentiation. These findings suggest that USF-1 and 2 play roles in C/EBPα expression, whereas the AP-4 represses it.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 2, 9 March 2007, Pages 517-521
نویسندگان
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