کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767452 | 1050733 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Upstream stimulatory factors regulate the C/EBPα gene during differentiation of 3T3-L1 preadipocytes
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کلمات کلیدی
USFAP-4EMSAC/EBP - C / EBPC/EBPα - C / EBPαElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزAdipocyte differentiation - تمایز آدپیوسیتیE-box - جعبه EUpstream stimulatory factor - فاکتور تحرک بالاDNA binding activity - فعالیت اتصال DNAwild type - نوع وحشیCCAAT enhancer binding protein - پروتئین اتصال دهنده تقویت کننده CCAATPromoter - پروموتر
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
During adipocyte differentiation, CCAAT/enhancer-binding protein α (C/EBPα) functions as a pleiotropic transcriptional activator of numerous adipocyte genes. The promoter of the C/EBPα gene has an E-box upstream of C/EBP binding site. Deletion or mutation of the E-box decreases promoter activity, suggesting that the E-box participates in the regulation of C/EBPα expression. Protein binding to the E-box during the adipocyte differentiation is increased as indicated by EMSA and UV cross-linking. Purification of the E-box binding proteins from differentiated 3T3-L1 adipocytes, showed that USF and AP-4 are associated with the E-box. Supershift analysis showed that USF1 and USF2 bind to this element as heterodimers, whereas the addition of anti-AP-4 antibody enhanced the binding complex, suggesting that AP-4 negatively regulates the promoter activity. The expression of AP-4 is reciprocally regulated with USF-1 during adipocyte differentiation. These findings suggest that USF-1 and 2 play roles in C/EBPα expression, whereas the AP-4 represses it.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 2, 9 March 2007, Pages 517-521
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 2, 9 March 2007, Pages 517-521
نویسندگان
Jae-woo Kim, Henrik Monila, Akhilesh Pandey, M. Daniel Lane,