کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767551 | 1050775 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A transgenic rat with the human ATTR V30M: A novel tool for analyses of ATTR metabolisms
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A transgenic rat with the human ATTR V30M: A novel tool for analyses of ATTR metabolisms A transgenic rat with the human ATTR V30M: A novel tool for analyses of ATTR metabolisms](/preview/png/10767551.png)
چکیده انگلیسی
Amyloidogenic transthyretin (ATTR) is the pathogenic protein of familial amyloidotic polyneuropathy (FAP). To establish a tool for analyses of ATTR metabolisms including after liver transplantations, we developed a transgenic rat model expressing human ATTR V30M and confirmed expressions of human ATTR V30M in various tissues. Mass spectrometry for purified TTR revealed that rat intrinsic TTR and human ATTR V30M formed tetramers. Congo red staining and immunohistochemistry revealed that nonfibrillar deposits of human ATTR V30M, but not amyloid deposits, were detected in the gastrointestinal tracts of the transgenic rats. At 24Â h after liver transplantation, serum human ATTR V30M levels in transgenic rats that received livers from normal rats became lower than detectable levels. These results thus suggest that this transgenic rat may be a useful animal model which analyzes the metabolism of human ATTR V30M including liver transplantation studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 352, Issue 2, 12 January 2007, Pages 299-304
Journal: Biochemical and Biophysical Research Communications - Volume 352, Issue 2, 12 January 2007, Pages 299-304
نویسندگان
Mitsuharu Ueda, Yukio Ando, Yoji Hakamata, Masaaki Nakamura, Taro Yamashita, Konen Obayashi, Shingo Himeno, Seiichiro Inoue, Yuki Sato, Takashi Kaneko, Nobutoki Takamune, Shogo Misumi, Shozo Shoji, Makoto Uchino, Eiji Kobayashi,