کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767706 | 1050795 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alachlor and carbaryl suppress lipopolysaccharide-induced iNOS expression by differentially inhibiting NF-κB activation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Nitric oxide (NO) produced by macrophages plays an important role in host defense and inflammation. We found that two agrochemicals, alachlor and carbaryl, inhibit lipopolysaccharide (LPS)-induced NO production by macrophages. In the present study, we investigated this inhibitory mechanism in RAW 264 cells. Both chemicals inhibited LPS-induced iNOS protein and mRNA expression as well as murine iNOS promoter activity. When treating these chemicals with reducing agents, the inhibition by carbaryl was reversed, but not the inhibition by alachlor. These chemicals also inhibited LPS-induced interferon-β (IFN-β) expression, an indispensable factor for LPS-induced iNOS expression. The inhibited iNOS expression, however, was not restored by exogenous IFN-β supplementation. LPS-induced nuclear translocation of NF-κB, which is necessary for the expression of IFN-β and iNOS, was inhibited by these chemicals: however, the LPS-induced degradation of IκB-α and IκB-β was inhibited only by alachlor. These results indicate that alachlor and carbaryl differentially impair the LPS-induced NF-κB activation, leading to the inhibition of NO production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 332, Issue 3, 8 July 2005, Pages 793-799
Journal: Biochemical and Biophysical Research Communications - Volume 332, Issue 3, 8 July 2005, Pages 793-799
نویسندگان
Mifumi Shimomura-Shimizu, Kei-ichi Sugiyama, Masashi Muroi, Ken-ichi Tanamoto,