کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767836 | 1050799 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of tissue transglutaminase by prolonged increase of intracellular Ca2+, but not by initial peak of transient Ca2+ increase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Regulation of tissue transglutaminase by prolonged increase of intracellular Ca2+, but not by initial peak of transient Ca2+ increase Regulation of tissue transglutaminase by prolonged increase of intracellular Ca2+, but not by initial peak of transient Ca2+ increase](/preview/png/10767836.png)
چکیده انگلیسی
Tissue transglutaminase (tTGase) is a member of calcium-dependent transamidation enzyme family, but a detailed regulation mechanism of tTGase by intracellular Ca2+ is not clearly understood. Arachidonic acid (AA) and maitotoxin (MTX) activated tTGase in a dose- and time-dependent manner. Transfection of tTGase siRNA largely inhibited tTGase expression and tTGase activation by MTX. AA induced an initial increase of intracellular Ca2+ followed by a prolonged increase. Removal of extracellular Ca2+ with EGTA blocked the prolonged Ca2+ increase in response to AA, although the initial Ca2+ increase remained. In contrast, EGTA completely blocked the increase of intracellular Ca2+ by MTX. The activation of tTGase by AA or MTX was significantly inhibited by EGTA. Moreover, EGTA prevented the prolonged increase of intracellular Ca2+ and tTGase activation by lysophosphatidic acid, but had no effect on the initial Ca2+ increase. These results suggested that tTGase is regulated by the prolonged increase of intracellular Ca2+ originated from Ca2+ influx, rather than by the initial peak of transient Ca2+ increase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 2, 18 November 2005, Pages 655-662
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 2, 18 November 2005, Pages 655-662
نویسندگان
Je-Ok Yoo, Sun-Ju Yi, Hyun Jung Choi, Woo Jin Kim, Young-Myeong Kim, Jeong-A. Han, Kwon-Soo Ha,