کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768051 | 1050803 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Promoter analysis of Mucor rouxii Î9-desaturase: Its implication for transcriptional regulation in Saccharomyces cerevisiae
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Promoter analysis of Mucor rouxii Î9-desaturase: Its implication for transcriptional regulation in Saccharomyces cerevisiae Promoter analysis of Mucor rouxii Î9-desaturase: Its implication for transcriptional regulation in Saccharomyces cerevisiae](/preview/png/10768051.png)
چکیده انگلیسی
Promoter study was performed to understand the transcriptional control of Î9-desaturase gene of Mucor rouxii. Several putative cis-elements involved in lipid metabolism were mapped by computational analysis. 5â² deletion analysis shows the presence of elements with repressing activity, especially in 122Â bp located upstream of the transcription start site. Truncation of these repressor domains showed that the promoter of M. rouxii is functional in Saccharomyces cerevisiae without additional components and is insensitive to nutritional depletion. The promoter also drove effectively the expression of a M. rouxii Î12-desaturase gene, and the linoleic acid content increased with the age of the yeast culture in parallel with the promoter activity. This approach provides a genetic tool for programming heterologous protein production in the yeast.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 335, Issue 2, 23 September 2005, Pages 400-405
Journal: Biochemical and Biophysical Research Communications - Volume 335, Issue 2, 23 September 2005, Pages 400-405
نویسندگان
Kobkul Laoteng, Supapon Cheevadhanarak, Morakot Tanticharoen, Bruno Maresca,