کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768327 | 1050807 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification of normal cellular prion protein from human platelets and the formation of a high molecular weight prion protein complex following platelet activation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Purification of normal cellular prion protein from human platelets and the formation of a high molecular weight prion protein complex following platelet activation Purification of normal cellular prion protein from human platelets and the formation of a high molecular weight prion protein complex following platelet activation](/preview/png/10768327.png)
چکیده انگلیسی
A method for the extraction and purification of PrPC, in its native monomeric form, from outdated human platelet concentrates is described. Both calcium ionophore platelet activation and lysis in Triton X-100 were evaluated as methods for the extraction of soluble platelet PrPC in its monomeric form. Following platelet activation, the majority of released PrPC was detected as a disulphide linked high molecular weight complex, which under reducing conditions could be separated into what appear to be stable non-disulphide linked PrP dimers or PrP covalently linked to another as yet unidentified protein. This phenomenon appears to be unique to activation since only monomeric PrPC was detected following lysis of resting platelets. Subsequently, PrPC was purified from the Triton X-100 lysate by sequential cation ion exchange and Cu2+ affinity chromatography. From 10Â L of outdated platelet concentrate, we were able to recover 1.29Â mg PrPC at a purity of 92%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 335, Issue 1, 16 September 2005, Pages 48-56
Journal: Biochemical and Biophysical Research Communications - Volume 335, Issue 1, 16 September 2005, Pages 48-56
نویسندگان
Michael Jones, Mark W. Head, John G. Connolly, Christine F. Farquhar, Valerie S. Hornsey, Duncan S. Pepper, Ian R. MacGregor,