کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10768345 1050807 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of a novel Cdc42 GEF that is localized to the PAT-3-mediated adhesive structure
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Identification of a novel Cdc42 GEF that is localized to the PAT-3-mediated adhesive structure
چکیده انگلیسی
In the model organism Caenorhabditis elegans, UNC-112 is colocalized with PAT-3/β-integrin and is a critical protein in the formation of PAT-3-mediated adhesive structure in body-wall muscle cells. However, the signaling pathway downstream of PAT-3/UNC-112 is largely unknown. To clarify the signaling pathway from PAT-3/UNC-112 to the actin cytoskeleton, we searched for and identified a novel Dbl homology/pleckstrin homology (DH/PH) domain containing protein, UIG-1 (UNC-112-interacting guanine nucleotide exchange factor-1). UIG-1 was colocalized with UNC-112 at dense bodies in body-wall muscle cells. UIG-1 showed CDC-42-specific GEF activity in vitro and induced filopodia formation in NIH 3T3 cells. Depletion of CDC-42 or PAT-3 in the developmental stage, by RNAi, prevented the formation of continuous actin filament in body-wall muscle cells. Taken together, these results suggest that UIG-1 links a PAT-3/UNC-112 complex to the CDC-42 signaling pathway during muscle formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 335, Issue 1, 16 September 2005, Pages 139-145
نویسندگان
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