کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768580 | 1050812 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning of the hamster p16 gene 5â² upstream region and its aberrant methylation patterns in pancreatic cancer
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The hamster model of pancreatic carcinogenesis is useful for understanding the development of human pancreatic cancer. However, there is only a small amount of hamster genetic information available for analyzing the gene alterations in hamster pancreatic cancers. Here, we determined the nucleotide sequence of the 5â² upstream region of the hamster p16 gene using a suppression polymerase chain reaction method combined with gene-specific primers. Based on this sequence, we analyzed the methylation status of the 5â² region by bisulfite sequencing in three normal pancreatic tissues and five pancreatic duct adenocarcinomas (PDAs). All five PDAs were highly methylated in the 5â² upstream region and showed reduced expressions of the p16 gene, while the three normal samples were demethylated. The method described in this study is a highly effective and rapid technique for determining the 5â² upstream region, and is applicable to epigenetic studies of the methylation status of this region.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 4, 12 August 2005, Pages 1249-1253
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 4, 12 August 2005, Pages 1249-1253
نویسندگان
Madoka Hanaoka, Kyoko Shimizu, Mayumi Shigemura, Ayumi Kato, Hiromasa Fujii, Kanya Honoki, Toshifumi Tsujiuchi,