کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10769557 1050823 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Age-dependent increase of etheno-DNA-adducts in liver and brain of ROS overproducing OXYS rats
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Age-dependent increase of etheno-DNA-adducts in liver and brain of ROS overproducing OXYS rats
چکیده انگلیسی
Reactive oxygen species (ROS) and lipid peroxidation (LPO) play a role in aging and degenerative diseases. To correlate oxidative stress and LPO-derived DNA damage, we determined etheno-DNA-adducts in liver and brain from ROS overproducing OXYS rats in comparison with age-matched Wistar rats. Liver DNA samples from 3- and 15-month-old OXYS and Wistar rats were analyzed for 1,N6-ethenodeoxyadenosine (εdA) and 3,N4-ethenodeoxycytidine (εdC) by immunoaffinity/32P-postlabelling. While εdA and εdC levels were not different in young rats, adduct levels were significantly higher in old OXYS rats when compared to old Wistar or young OXYS rats. Frozen rat brain sections were analyzed for εdA by immunostaining of nuclei. Brains from old OXYS rats accumulated εdA more frequently than age-matched Wistar rats. Our results demonstrate increased LPO-induced DNA damage in organs of OXYS rats which correlates with their known shorter life-span and elevated frequency of chronic degenerative diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 336, Issue 2, 21 October 2005, Pages 478-482
نویسندگان
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