کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10769760 1050825 2005 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A point mutation at phenylalanine 663 abolishes protein kinase Cα's ability to translocate to the perinuclear region and activate phospholipase D1
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A point mutation at phenylalanine 663 abolishes protein kinase Cα's ability to translocate to the perinuclear region and activate phospholipase D1
چکیده انگلیسی
Previous research showed that protein kinase Cα (PKCα) translocated to the perinuclear region and activated phospholipase D1, but the mechanism involved was not clear. Here, we provide evidence that Phe 663 (the 10th amino acid from C-terminus) of PKCα is essential for its translocation. A point mutation (F663D) completely blocked PKCα's binding to and activation of phospholipase D1. Further studies showed that deletion of the C-terminal nine amino acids of PKCα did not alter its translocation to the perinuclear region but deletion of the C-terminal 10 amino acids and the F663D mutation abolished this translocation. The F663D mutant was found to be resistant to dephosphorylation, which might account for its inability to translocate to the perinuclear region and activate PLD1, since dephosphorylation of PKCα is required for its relocation from plasma membrane to the perinuclear region.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 3, 5 August 2005, Pages 750-753
نویسندگان
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