کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10769762 | 1050825 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the organic cation transporter SLC22A16: A doxorubicin importer
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization of the organic cation transporter SLC22A16: A doxorubicin importer Characterization of the organic cation transporter SLC22A16: A doxorubicin importer](/preview/png/10769762.png)
چکیده انگلیسی
Specific efflux transporters, such as P-glycoprotein, have been shown to confer drug resistance by decreasing the intracellular accumulation of anticancer drugs. Understanding influx transporters, as well as efflux transporters, is essential to overcome this resistance. We report the expression profile and pharmacological characterization of an organic cation transporter, SLC22A16. The results of our experiments indicate that SLC22A16 is a mediator of doxorubicin uptake in cancer cells. Quantitative real-time RT-PCR analyses show that SLC22A16 is expressed in primary samples taken from patients with acute leukemia. Xenopus oocytes injected with SLC22A16 cRNA import doxorubicin, a widely used anticancer drug for hematological malignancies, in a saturable and dose-dependent manner. The apparent Km value for doxorubicin import was 5.2 ± 0.4 μM. In cytotoxic assays, stable transfectants of leukemic Jurkat cells overexpressing SLC22A16 cells became significantly more sensitive to doxorubicin (2 μM) treatment. Characterization of SLC22A16 will help in designing novel therapies targeting hematological malignancies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 3, 5 August 2005, Pages 754-762
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 3, 5 August 2005, Pages 754-762
نویسندگان
Mitsunori Okabe, Michiaki Unno, Hideo Harigae, Mitsuo Kaku, Yoko Okitsu, Takeshi Sasaki, Takayuki Mizoi, Kenichi Shiiba, Hitomi Takanaga, Tetsuya Terasaki, Seiki Matsuno, Iwao Sasaki, Sadayoshi Ito, Takaaki Abe,