کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10770070 | 1050828 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The yeast multicopper oxidase Fet3p and the iron permease Ftr1p physically interact
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: The yeast multicopper oxidase Fet3p and the iron permease Ftr1p physically interact The yeast multicopper oxidase Fet3p and the iron permease Ftr1p physically interact](/preview/png/10770070.png)
چکیده انگلیسی
High affinity iron uptake in yeast is carried out by a multicomponent system formed by the ferroxidase Fet3p and the iron permease Ftr1p. The currently accepted model predicts that Fet3p and Ftr1p are functionally associated, however, a structural interaction between these two proteins has not been proven yet. The methylotrophic yeast Pichia pastoris has been used to perform cross-linking studies aimed to demonstrate the existence of a Fet3p-Ftr1p complex. Cross-linking of membrane suspensions with the membrane-impermeable reagents DTSSP and BS3 has evidenced the presence of a high molecular weight band with Fet3p oxidase activity. This band has been purified and subjected to N-terminal sequence analysis. Two sequences were found in the cross-linked species, one of which could be assigned to Fet3p and the other to Ftr1p. This is the first experimental demonstration that Fet3p and Ftr1p are physically associated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 2, 29 July 2005, Pages 432-437
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 2, 29 July 2005, Pages 432-437
نویسندگان
M. Carmela Bonaccorsi di Patti, Rossella Miele, M. Eugenia Schininà , Donatella Barra,