کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10770173 1050830 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interaction of hRad51 and hRad52 with MCM complex: A cross-talk between recombination and replication proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Interaction of hRad51 and hRad52 with MCM complex: A cross-talk between recombination and replication proteins
چکیده انگلیسی
Human Rad51 and Rad52 are implicated in DNA repair during replication. Here we show, by pull-down assays, that purified hRad51 and hRad52 interact with each other as well as with Mini chromosome maintenance (MCM) proteins in HeLa cell extracts. Furthermore, immunoprecipitation experiments corroborate the same where hRad51 and hRad52 proteins not only cross-talk with each other but also pull down MCM3 and MCM2/3 proteins, respectively. The interaction scoring assays, performed reciprocally, demonstrate the same specificity, based on which, we speculate that MCM complex exhibits strong propensity to get physically recruited to the sites where hRad51 and hRad52-mediated homologously aligned ends need to be replicationally repaired.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 329, Issue 4, 22 April 2005, Pages 1240-1245
نویسندگان
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