کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10771134 | 1050838 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intracellular interferon triggers Jak/Stat signaling cascade and induces p53-dependent antiviral protection
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Intracellular interferons (IFNs) exert biological functions similar to those of extracellular IFNs, but the signal transduction pathway triggered by the intracellular ligands has not been fully revealed. We investigated the signaling cascade by sequence-specific knockdown of signaling molecules by means of the RNA interference. Truncated IFN-β gene was constructed so that the N-terminal secretory signal sequence was deleted (SD.IFN-β). Cells transfected with this construct showed phosphorylation and activation of the STAT1 without any detectable secretion of the cytokine. The MHC class I expression was significantly augmented, while the augmentation was suppressed by short interfering RNA duplexes specific for JAK1, TYK2, and IFN-α/β receptor (IFNAR) 1 and 2c chains. The SD.IFN-β also induced p53 and phosphorylation of p53 at Ser15. Specific silencing of p53 abrogated the antiviral effect of SD.IFN-β, suggesting that the tumor suppressor is critically involved in antiviral defense mediated by intracellular IFN.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 329, Issue 3, 15 April 2005, Pages 1139-1146
Journal: Biochemical and Biophysical Research Communications - Volume 329, Issue 3, 15 April 2005, Pages 1139-1146
نویسندگان
Masaharu Shin-Ya, Hideyo Hirai, Etsuko Satoh, Tsunao Kishida, Hidetsugu Asada, Fumiko Aoki, Masako Tsukamoto, Jiro Imanishi, Osam Mazda,