کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10771264 | 1050840 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
1-Butanol interferes with phospholipase D1 and protein kinase Cα association and inhibits phospholipase D1 basal activity
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: 1-Butanol interferes with phospholipase D1 and protein kinase Cα association and inhibits phospholipase D1 basal activity 1-Butanol interferes with phospholipase D1 and protein kinase Cα association and inhibits phospholipase D1 basal activity](/preview/png/10771264.png)
چکیده انگلیسی
1-Butanol is commonly used as a substrate for phospholipase D (PLD) activity measurement. Surprisingly we found that, in the presence of 30 mM 1-butanol (standard PLD assay conditions), PLD1 activity in COS-7 cells was lost after incubation for 2 min. In contrast, in the presence of the protein kinase C (PKC) inhibitor staurosporine or dominant negative PKCα D481E, the activity was sustained for at least 30 min. The binding between PLD1 and PKCα was also lost after 2 min incubation with 30 mM 1-butanol while staurosporine and D481E maintained the binding. 1-Butanol at 2 mM did not inhibit PLD1 basal activity or PLD1 binding to PKCα, and staurosporine and PKCα D481E produced a constant increase in PLD1 basal activity of 2-fold. These results indicate that 1-butanol is inhibitory to PLD1 activity by reducing its association with PKCα, and that the concentration of 1-butanol is an important consideration in assaying basal PLD1 activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 4, 25 February 2005, Pages 1047-1051
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 4, 25 February 2005, Pages 1047-1051
نویسندگان
Tianhui Hu, John H. Exton,