کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10771379 | 1050841 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Specific inactivation of cysteine protease-type cathepsin by singlet oxygen generated from naphthalene endoperoxides
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Specific inactivation of cysteine protease-type cathepsin by singlet oxygen generated from naphthalene endoperoxides Specific inactivation of cysteine protease-type cathepsin by singlet oxygen generated from naphthalene endoperoxides](/preview/png/10771379.png)
چکیده انگلیسی
Singlet oxygen is a causal factor in light-induced skin photoaging and the cytotoxic process of tumor cells in photodynamic chemotherapy. To develop a better understanding of the functional consequences of protein modification by singlet oxygen, the effects of naphthalene endoperoxide on lysosomal protease, cathepsin, were examined. When the soluble fraction of normal human fetal skin fibroblast cells was treated with the endoperoxide, the activities of cysteine proteases, cathepsins B and L/S, were inhibited, but that of aspartate protease, cathepsin D/E, was not. The reduction of the endoperoxide-treated soluble fractions by treatment with dithiothreitol barely recovered the activities. Cathepsin B, purified from normal human liver, exhibited similar profiles to that in cytosol. These data suggest that singlet oxygen oxidatively modifies an amino acid residue essential for catalysis and consequently results in the irreversible inactivation of cysteine protease-type cathepsin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 331, Issue 1, 27 May 2005, Pages 215-223
Journal: Biochemical and Biophysical Research Communications - Volume 331, Issue 1, 27 May 2005, Pages 215-223
نویسندگان
Yuki Nagaoka, Kaoru Otsu, Futoshi Okada, Kazuaki Sato, Yoshihiro Ohba, Naoki Kotani, Junichi Fujii,