کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10771584 | 1050843 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of DNase γ in the resected double-strand DNA breaks in immunoglobulin genes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Somatic hypermutation (SHM) of immunoglobulin variable (V) region genes occurs in the germinal center (GC) B cells during immune responses, depending on activation-induced cytidine deaminase (AID). SHM is associated with resected double-strand DNA breaks (DSBs) which were shown to occur specifically in rearranged V regions in the GC B cells and CD40-stimulated B cells expressing AID. So far, endonucleases responsible for the DSBs have not been identified. Here we show that DNase γ, a member of DNase I family of endonucleases, is expressed in GC B cells and CD40-stimulated B cells. Overexpression of DNase γ in the mutation-competent Ramos B-cell line resulted in a marked increase in the resected but not blunt DSBs in the V region. Conversely, a selective DNase γ inhibitor, DR396, suppressed the generation of the resected DSBs. These results suggest that DNase γ is involved in the generation of resected DSBs associated with SHM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 1, 4 February 2005, Pages 76-83
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 1, 4 February 2005, Pages 76-83
نویسندگان
Mariko Okamoto, Noriaki Okamoto, Hisako Yashiro, Daisuke Shiokawa, Satoshi Sunaga, Atsushi Yoshimori, Sei-ichi Tanuma, Daisuke Kitamura,