کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10795849 1052606 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Product-controlled steady-state kinetics between cytochrome aa3 from Rhodobacter sphaeroides and equine ferrocytochrome c analyzed by a novel spectrophotometric approach
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Product-controlled steady-state kinetics between cytochrome aa3 from Rhodobacter sphaeroides and equine ferrocytochrome c analyzed by a novel spectrophotometric approach
چکیده انگلیسی
► Steady state kinetics of R. sphaeroides cyt c oxidase and horse cyt c is monophasic. ► Full spectrum deconvolution used to obtain [cyt c2+] and [cyt c3+] at each point. ► Analysis shows product inhibition by cyt c3+ due to interaction at two sites. ► cyt c3+ is a competitive inhibitor at the catalytic site. ► cyt c3+ also binds at a second, allosteric site and modulates both KM and Vmax.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1817, Issue 10, October 2012, Pages 1894-1900
نویسندگان
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