کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10797327 1053214 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism
چکیده انگلیسی
► The intact purified FsrC membrane histidine kinase was studied by SRCD spectroscopy. ► Far-UV spectra revealed that FsrC is 61% α-helical and relatively thermostable. ► Tertiary structure was found to be significantly affected upon GBAP ligand binding. ► Titration experiments revealed a calculated kd value of 2 μM. ► SRCD provides a valuable screening tool for membrane protein stability under a range of detergent conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1818, Issue 7, July 2012, Pages 1595-1602
نویسندگان
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