Structure and internal organization of overcharged cationic-lipid/peptide/DNA self-assembly complexes

► Lamellar lipid/ε-peptide/DNA complex contains DNA monolayer and lipid bilayer. ► ε-peptides insert between parallel DNA and are present on particle surface. ► DNA-DNA distance (dDNA) depends on total positive to negative charge ratio. ► The dependence is explained by an electrostatic analysis. ► Variation of surface potential has a similar tendency as the change in dDNA.