Lipid-mediated membrane binding properties of Disabled-2

► Reconstitution of Dab2 N-PTB in sulfatide- and PtdIns(4,5)P2-enriched micelles. ► Sulfatide- and PtdIns(4,5)P2-binding sites overlap in Dab2 N-PTB. ► Sulfatides, but not PtdIns(4,5)P2, induce Dab2 N-PTB structural changes. ► Sulfatides, but not PtdIns(4,5)P2, contribute to membrane insertion of Dab2 N-PTB.