Solution structure of subunit F (Vma7p) of the eukaryotic V1VO ATPase from Saccharomyces cerevisiae derived from SAXS and NMR spectroscopy

►First low resolution structure of subunit F of a eukaryotic V-ATPase. ►Shape of the truncated form of F, F1-94, allows the localization of the N- and C-termini in the entire protein. ►NMR solution structure of C-terminal peptide, F90-116. ►New insight into the static and mechanistic properties of subunit F inside the V1VO ATPase.