کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10798139 | 1053299 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
N-acyl phosphatidylethanolamines affect the lateral distribution of cholesterol in membranes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CTLd-erythro-N-palmitoyl-sphingomyelin1-palmitoyl-2-stearoyl-(7-doxyl)-sn-glycero-3-phosphocholine7SLPCcholestatrienolN-acyl phosphatidylethanolamineDPHPoPCPSMβ-Cyclodextrin1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine - 1-پالمیتویل-2-اولئویل-اس-گلیسرو-3-فسفاتانولامین1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine - 1-پالمیتویل-2-اولئویل-اسن-گلیسرو-3-فسفوشولین1,6-diphenyl-1,3,5-hexatriene - 1،6-دیفنیل-1،3،5-هگزیدریلMembrane domain - دامنه غشاءNAPE - ناپاPOPE - پاپcholesterol - کلسترولQuenching - کوئنچ کردن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: N-acyl phosphatidylethanolamines affect the lateral distribution of cholesterol in membranes N-acyl phosphatidylethanolamines affect the lateral distribution of cholesterol in membranes](/preview/png/10798139.png)
چکیده انگلیسی
N-Acyl phosphatidylethanolamines are negatively charged phospholipids, which are naturally occurring albeit at low abundance. In this study, we have examined how the amide-linked acyl chain affected the membrane behavior of the N-acyl-1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine (N-acyl-POPE) or N-acyl-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (N-acyl-DPPE), and how the molecules interacted with cholesterol. The gel â liquid crystalline transition temperature of sonicated N-acyl phosphatidylethanolamine vesicles in water correlated positively with the number of palmitic acyl chains in the molecules. Based on diphenylhexatriene steady state anisotropy measurements, the presence of 33 mol% cholesterol in the membranes removed the phase transition from N-oleoyl-POPE bilayers, but failed to completely remove it from N-palmitoyl-DPPE and N-palmitoyl-POPE bilayers, suggesting rather weak interaction of cholesterol with the N-saturated NAPEs. The rate of cholesterol desorption from mixed monolayers containing N-palmitoyl-DPPE and cholesterol (1:1 molar ratio) was much higher compared to cholesterol/DPPE binary monolayers, suggesting a weak cholesterol interaction with N-palmitoyl-DPPE also in monolayers. In bilayer membranes, both N-palmitoyl-POPE and N-palmitoyl-DPPE failed to form sterol-rich domains, and in fact appeared to displace sterol from sterol/N-palmitoyl-sphingomyelin domains. The present data provide new information about the effects of saturated NAPEs on the lateral distribution of cholesterol in NAPE-containing membranes. These findings may be of relevance to neural cells which accumulate NAPEs during stress and cell injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1715, Issue 1, 30 August 2005, Pages 49-56
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1715, Issue 1, 30 August 2005, Pages 49-56
نویسندگان
Bohdana Térová, Gitte Petersen, Harald S. Hansen, J. Peter Slotte,