کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799162 | 1054236 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adipose induces myoblast differentiation and mediates TNFα-regulated myogenesis
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کلمات کلیدی
TNFαTPRsTetratricopeptide repeatsMrf4KLF15Myf5MyogeninKLFsMyogenesisADPCREBHDACMEF2GAPDHPPARC/EBP - C / EBPMRFs - MRF هاTranscriptional regulation - تنظیم ترانزیتیtumor necrosis factor alpha - تومور نکروز عامل آلفاMyosin heavy chain - زنجیره سنگین میوزینmyocyte enhancer factor-2 - عامل افزایش دهنده myocyte-2Myogenic regulatory factors - عوامل کنترل کننده MyogenicMHC - مجموعه سازگاری بافتی اصلیMYOG - منوhistone deacetylase - هیستون داستیلازCcaat-enhancer-binding protein - پروتئین اتصال دهنده تقویت کننده CcaatcAMP-responsive element binding protein - پروتئین اتصال دهنده عنصر پاسخ دهنده cAMPAdipose - چربیglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازperoxisome proliferator-activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Skeletal muscle formation is controlled by multiple processes. These processes are tightly regulated by muscle regulatory factors. Genes that are highly and specifically expressed during myogenesis need to be identified. In the present study, the role of an anti-adipogenic gene adipose (Adp) in myogenesis is demonstrated. We discover that the expression of Adp is increased during myoblast differentiation. Overexpression of Adp in mouse myoblast C2C12 cells leads to an increase of myogenesis and up-regulation of MyoG expression. The inhibition effect of tumor necrosis factor α (TNFα) on myogenic differentiation is reversed by Adp-overexpression. Further research showed that TNFα significantly decreases Adp expression at both the mRNA and protein levels. Luciferase reporter assays showed that TNFα can inhibit Adp gene promoter activity and impair gene transcription. KLF15 was found to regulate the transcription of Adp. Furthermore, the expression of KLF15 and its binding to Adp promoter were reduced due to TNFα treatment. The reduced KLF15 expression after TNFα treatment is responsible for the repression of Adp gene promoter activity. KLF15 was also found to participate in Adp-mediated myogenic differentiation. Taken together, these data identify Adp as a positive modulator of myoblast differentiation and provide new insights for Adp function research.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1839, Issue 11, November 2014, Pages 1183-1195
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1839, Issue 11, November 2014, Pages 1183-1195
نویسندگان
Yanling Wu, Qinqiang Long, Zifeng Zheng, Qiao Xia, Fenyun Wen, Xiaoyue Zhu, Xiaoling Yu, Zaiqing Yang,