کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799853 | 1054596 | 2016 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An in vitro evaluation of epigallocatechin gallate (eGCG) as a biocompatible inhibitor of ricin toxin
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
FCSBCAEGCG3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideBSA - BSADMSO - DMSOPMA - LDC هاMTT - MTTbovine serum albumin - آلبومین سرم گاوEndocytosis - آندوسیتوز یا درون بریepigallocatechin gallate - اپی گالوستاچین گالاتbicinchoninic acid - بیسینکنینیک اسیدstandard error of the mean - خطای استاندارد میانگینDimethyl sulfoxide - دیمتیل سولفواکسیدcircular dichroism - رنگ تابی دورانیfetal calf serum - سرم گوساله جنینendoplasmic reticulum - شبکه آندوپلاسمی SEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیPolyphenol - پلی فنلTEA - چای
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The catechin, epigallocatechin gallate (eGCG), found in green tea, has inhibitory activity against a number of protein toxins and was investigated in relation to its impact upon ricin toxin (RT) in vitro. The IC50 for RT was 0.08 ± 0.004 ng/mL whereas the IC50 for RT + 100 μM eGCG was 3.02 ± 0.572 ng/mL, indicating that eGCG mediated a significant (p < 0.0001) reduction in ricin toxicity. This experiment was repeated in the human macrophage cell line THP-1 and IC50 values were obtained for RT (0.54 ± 0.024 ng/mL) and RT + 100 μM eGCG (0.68 ± 0.235 ng/mL) again using 100 μM eGCG and was significant (p = 0.0013). The documented reduction in ricin toxicity mediated by eGCG was found to be eGCG concentration dependent, with 80 and 100 μg/mL (i.e. 178 and 223 μM respectively) of eGCG mediating a significant (p = 0.0472 and 0.0232) reduction in ricin toxicity at 20 and 4 ng/ml of RT in Vero and THP-1 cells (respectively). When viability was measured in THP-1 cells by propidium iodide exclusion (as opposed to the MTT assays used previously) 10 ng/mL and 5 ng/mL of RT was used. The addition of 1000 μM and 100 μM eGCG mediated a significant (p = 0.0015 and < 0.0001 respectively) reduction in ricin toxicity relative to an identical concentration of ricin with 1 μg eGCG. Further, eGCG (100 μM) was found to reduce the binding of RT B chain to lactose-conjugated Sepharose as well as significantly (p = 0.0039) reduce the uptake of RT B chain in Vero cells. This data suggests that eGCG may provide a starting point to refine biocompatible substances that can reduce the lethality of ricin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1860, Issue 7, July 2016, Pages 1541-1550
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1860, Issue 7, July 2016, Pages 1541-1550
نویسندگان
Paul D.R. Dyer, Arun K. Kotha, Alex S. Gollings, Susan A. Shorter, Thomas R. Shepherd, Marie W. Pettit, Bruce D. Alexander, Giulia T.M. Getti, Samer El-Daher, Les Baillie, Simon C.W. Richardson,