کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801733 | 1055632 | 2016 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transglutaminase type 2-dependent selective recruitment of proteins into exosomes under stressful cellular conditions
ترجمه فارسی عنوان
غلظت پروتئین وابسته به نوع 2 متابولیسم ترومبوکلئوتامیناز به اگزوزوم ها در شرایط سلولی تنش زا
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کلمات کلیدی
MEFBAPHEK293TG2ESCRTmHTTMG132BAG3TSG101Alix - آلیکسExosomes - اگزوزوم هاHuntington's disease - بیماری هانتینگتونhuman embryonic kidney cells - سلول های کلیوی جنینی انسانmurine embryonic fibroblasts - فیبروبلاست های جنینی جنینیendosomal sorting complex required for transport - مجتمع مرتب سازی آندوسومی مورد نیاز برای حمل و نقلTumor susceptibility gene 101 - ژن 101 حساسیت تومور
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Numerous studies are revealing a role of exosomes in intercellular communication, and growing evidence indicates an important function for these vesicles in the progression and pathogenesis of cancer and neurodegenerative diseases. However, the biogenesis process of exosomes is still unclear. Tissue transglutaminase (TG2) is a multifunctional enzyme with different subcellular localizations. Particularly, under stressful conditions, the enzyme has been also detected in the extracellular matrix, but the mechanism(s) by which TG2 is released outside the cells requires further investigation. Therefore, the goal of the present study was to determine whether exosomes might be a vehicle for TG2 to reach the extracellular space, and whether TG2 could be involved in exosomes biogenesis. To address this issue, we isolated and characterized exosomes derived from cells either expressing or not TG2, under stressful conditions (i.e. proteasome impairment or expressing a mutated form of huntingtin (mHtt) containing 84 polyglutamine repeats). Our results show that TG2 is present in the exosomes only upon proteasome blockade, a condition in which TG2 interacts with TSG101 and ALIX, two key proteins involved in exosome biogenesis. Interestingly, we found that TG2 favours the assembly of a protein complex including mHtt, ALIX, TSG101 and BAG3, a co-chaperone involved in the clearance of mHtt. The formation of this complex is paralleled by the selective recruitment of mHtt and BAG3 in the exosomes derived from TG2 proficient cells only. Overall, our data indicate that TG2 is an important player in the biogenesis of exosomes controlling the selectivity of their cargo under stressful cellular conditions. In addition, these vesicles represent the way by which cells can release TG2 into the extracellular space under proteostasis impairment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 8, August 2016, Pages 2084-2092
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 8, August 2016, Pages 2084-2092
نویسندگان
Laura Diaz-Hidalgo, Sara Altuntas, Federica Rossin, Manuela D'Eletto, Claudia Marsella, Maria Grazia Farrace, Laura Falasca, Manuela Antonioli, Gian Maria Fimia, Mauro Piacentini,