کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801766 | 1055635 | 2016 | 59 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The signaling module cAMP/Epac/Rap1/PLCε/IP3 mobilizes acrosomal calcium during sperm exocytosis
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کلمات کلیدی
PLC8-pCPT-2′-O-Me-cAMP2-aminoethoxydiphenylborateGEFEpac2-APBIP3pKaGSTSACPBSPVPHTFIPTG1,4,5-trisphosphate - 1،4،5-تری فسفاتcAMP - cAMPSDS–PAGE - SDS-PAGECyclic adenosine monophosphate - آدنوزین مونوفسفات CyclicStreptolysin O - استرپتولیسین Oslo - اسلوsodium dodecyl sulfate–polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدProtein transduction - انتقال پروتئینExocytosis - اگزوسیتوزisopropyl-β-d-thio-galactoside - ایزوپروپیل بتا-تیو گالاکتوزیدguanine nucleotide exchange factor - فاکتور تبادل نوکلئوتید گوانینphospholipase C - فسفولیپاز CPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریacrosome reaction - واکنش آکروزومcAMP-dependent protein kinase - پروتئین کیناز وابسته به cAMPpolyvinylpyrrolidone - پلی وینیل پیرولیدونCalcium - کلسیمglutathione-S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The signaling module cAMP/Epac/Rap1/PLCε/IP3 mobilizes acrosomal calcium during sperm exocytosis The signaling module cAMP/Epac/Rap1/PLCε/IP3 mobilizes acrosomal calcium during sperm exocytosis](/preview/png/10801766.png)
چکیده انگلیسی
Exocytosis of the sperm's single secretory granule, or acrosome, is a regulated exocytosis triggered by components of the egg's investments. In addition to external calcium, sperm exocytosis (termed the acrosome reaction) requires cAMP synthesized endogenously and calcium mobilized from the acrosome through IP3-sensitive channels. The relevant cAMP target is Epac. In the first part of this paper, we present a novel tool (the TAT-cAMP sponge) to investigate cAMP-related signaling pathways in response to progesterone as acrosome reaction trigger. The TAT-cAMP sponge consists of the cAMP-binding sites of protein kinase A regulatory subunit RIβ fused to the protein transduction domain TAT of the human immunodeficiency virus-1. The sponge permeated into sperm, sequestered endogenous cAMP, and blocked exocytosis. Progesterone increased the population of sperm with Rap1-GTP, Rab3-GTP, and Rab27-GTP in the acrosomal region; pretreatment with the TAT-cAMP sponge prevented the activation of all three GTPases. In the second part of this manuscript, we show that phospholipase Cε (PLCε) is required for the acrosome reaction downstream of Rap1 and upstream of intra-acrosomal calcium mobilization. Last, we present direct evidence that cAMP, Epac, Rap1, and PLCε are necessary for calcium mobilization from sperm's secretory granule. In summary, we describe here a pathway that connects cAMP to calcium mobilization from the acrosome during sperm exocytosis. Never before had direct evidence for each step of the cascade been put together in the same study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 4, April 2016, Pages 544-561
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 4, April 2016, Pages 544-561
نویسندگان
Ornella Lucchesi, MarÃa C. Ruete, MatÃas A. Bustos, MarÃa F. Quevedo, Claudia N. Tomes,