کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10802362 | 1055686 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A role for miR-142-3p in colony-stimulating factor 1-induced monocyte differentiation into macrophages
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کلمات کلیدی
CSF1miR-142-3pNAB2SPI-1TAB2Gfi-1CMMLMafBNR3C1KLF4RTKM-CSFCUX1Egr2Rac1PI3KcAMP - cAMPRas-related C3 botulinum toxin substrate 1 - زیر بغل سم بوتولینوم C3 مرتبط با ریز 1colony-stimulating factor 1 - عامل تحریک کننده کلنی 1Krüppel-like factor 4 - عامل کریپال مانند 4Phosphatidyl Inositol 3-kinase - فسفاتیدیل آنزیم 3-کینازchronic myelomonocytic leukemia - لوسمی میلوومونوسیتی مزمنmacrophage-colony stimulating factor - ماکروفاژ - کلنی عامل تحریک کنندهMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAEarly growth response 2 - پاسخ رشد اولیه 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The differentiation of human peripheral blood monocytes into macrophages can be reproduced ex vivo by culturing the cells in the presence of colony-stimulating factor 1 (CSF1). Using microarray profiling to explore the role of microRNAs (miRNAs), we identified a dramatic decrease in the expression of the hematopoietic specific miR-142-3p. Up- and down-regulation of this miRNA in primary human monocytes altered CSF1-induced differentiation of monocytes, as demonstrated by changes in the expression of the cell surface markers CD16 and CD163. One of the genes whose expression is repressed by miR-142-3p encodes the transcription factor Early Growth Response 2 (Egr2). In turn, Egr2 associated with its co-repressor NGFI-A (Nerve Growth Factor-Induced gene-A) binding protein 2 (NAB2) binds to the pre-miR-142-3p promoter to negatively regulate its expression. Interestingly, the expression of miR-142-3p is abnormally low in monocytes from patients with the most proliferative forms of chronic myelomonocytic leukemia (CMML), and miR-142-3p re-expression in CMML dysplastic monocytes can improve their differentiation potential. Altogether, miR-142-3p which functions in a molecular circuitry with Egr2 is an actor of CSF1-induced differentiation of human monocytes whose expression could be altered in CMML.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1833, Issue 8, August 2013, Pages 1936-1946
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1833, Issue 8, August 2013, Pages 1936-1946
نویسندگان
Brice Lagrange, Romain Z. Martin, Nathalie Droin, Romain Aucagne, Jérôme Paggetti, Anne Largeot, Raphaël Itzykson, Eric Solary, Laurent Delva, Jean-Noël Bastie,