PP2A phosphatase suppresses function of the mesenchymal invasion regulator NEDD9

► PP2A phosphatase regulates 105 kDa NEDD9 phosphoform. ► PP2A interacts with the Serine-Rich region (SRR) of NEDD9. ► Constitutive phosphorylation of NEDD9 S369 in the SRR promotes cell spreading. ► The tumour suppressor PP2A suppresses NEDD9-mediated spreading and switch to mesenchymal morphologies.