Residual structure and dynamics in DMSO-d6 denatured Dynein Light Chain protein

► Residue level characterization of the DMSO-d6 denatured ensemble of DLC8 with NMR. ► Presence of slow conformational exchange in the denatured ensemble. ► Native helical propensities in the segment Ser 14- His 55. ► High conformational exchange in the segments corresponding to native helices. ► Two helices are supposedly formed first during folding followed by β2 and β5.