Maize Ac/Ds transposon system leads to highly efficient germline transmission of transgenes in medaka (Oryzias latipes)

► Maize Ac/Ds enhances transient transgenic expression and germline transmission in medaka. ► Maize Ac/Ds causes multiple transgenic insertions in transgenic medaka. ► Two stable transgenic medaka lines were generated with inducible GFP expression. ► Tg(hsp70:gfp) transgenic medaka showed induced GFP expression by heat shock. ► Tg(cyp1a1:gfp) transgenic medaka showed induced GFP expression by TCDD.