کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10814982 | 1058438 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PPIP5K1 interacts with the exocyst complex through a C-terminal intrinsically disordered domain and regulates cell motility
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کلمات کلیدی
DIPPinositol pyrophosphatesInositol pyrophosphatePIP5KSNARESBDRhoACBDGSTArp2/3 - Arp2 / 3inositol 1,4,5-trisphosphate - inositol 1،4،5-trisphosphatePtdIns(4,5)P2 - PtdIns (4،5) P2siRNA - siRNAIntrinsic disorder - اختلال ذهنیExocyst - اگزوسیستTev - به توRalA - خرگوشCalmodulin binding domain - دامنه اتصال کالمدولینRas homolog gene family member A - عضو خانواده خانواده ژن رای همولوگPhosphatidylinositol 4-phosphate 5-kinase - فسفاتیدیلینواستیل 4-فسفات 5-کینازCell migration - مهاجرت سلولیhemagglutinin - هماگلوتینینPleckstrin Homology - همخوانی PleckstrinTobacco etch virus - ویروس تنباکو اچProteomics - پروتئومیکسglutathione-S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cellular signaling involves coordinated regulation of many events. Scaffolding proteins are crucial regulators of cellular signaling, because they are able to affect numerous events by coordinating specific interactions among multiple protein partners in the same pathway. Scaffolding proteins often contain intrinsically disordered regions (IDR) that facilitate the formation and function of distinct protein complexes. We show that PPIP5K1 contains an unusually long and evolutionarily conserved IDR. To investigate the biological role(s) of this domain, we identified interacting proteins using affinity purification coupled with mass spectrometry. Here, we report that PPIP5K1 is associated with a network of proteins that regulate vesicle-mediated transport. We further identified exocyst complex component 1 as a direct interactor with the IDR of PPIP5K1. Additionally, we report that knockdown of PPIP5K1 decreases motility of HeLa cells in a wound-healing assay. These results suggest that PPIP5K1 might play an important role in regulating function of exocyst complex in establishing cellular polarity and directional migration of cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 28, Issue 5, May 2016, Pages 401-411
Journal: Cellular Signalling - Volume 28, Issue 5, May 2016, Pages 401-411
نویسندگان
Gayane Machkalyan, Phan Trieu, Darlaine Pétrin, Terence E. Hébert, Gregory J. Miller,