PLCβ isoforms differ in their subcellular location and their CT-domain dependent interaction with Gαq

► The subcellular location of PLCβ varies in an isoform-specific manner. ► PLCβ1 and PLCβ4 are enriched at the plasma membrane. ► PLCβ2 and PLCβ3 are enriched in the cytosol. ► Recruitment of PLCβ3 to the plasma membrane can be triggered by active Gαq. ► CT domains derived from the various isoforms differ in their interaction with Gαq.