کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815500 | 1058476 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CD1d expressed in mast cell surface enhances IgE production in B cells by up-regulating CD40L expression and mediator release in allergic asthma in mice
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کلمات کلیدی
LTSTIMPsCD1dPLA2CCR2BMMCsTLRBALAPCOVAα-galCCL2IgEMmpsPCA - PCAα-galactosylceramide - α-گالاکتوسیلسرامیدAllergic asthma - آسم آلرژیکPassive cutaneous anaphylaxis - آنافیلاکسی پوستی منفعلAntigen presenting cells - آنتیژن ارائه سلولphospholipase A2 - آنزیم فسفولیپاز A2 periodic acid-Schiff - اسید فسفریک SchiffOvalbumin - اوبلبومینtoll like receptor - تلفات مانند گیرندهiNKT cells - سلول های iNKTBone marrow-derived mast cells - سلول های مشتق شده مغز استخوانbronchoalveolar lavage - لارو برونکلو آلوئولارLeukotrienes - لکوتری هاchemokine ligand 2 - لیگاند شیمیایی 2Mast cells - ماست سل هاMatrix metalloproteinases - متالوپروتئیناز ماتریکسPAS - نهchemokine receptor 2 - گیرنده شیمیایی 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Mast cells play important roles via FcεRI-mediated activation in allergic asthma. A nonpolymorphic MHC I-like molecule CD1d, which is mainly expressed in APCs, presents glycolipid Ag to iTCR on iNKT cells and modulates allergic responses. This study aimed to investigate the role of CD1d on IgE production and mast cell activation related to allergic asthma. Bone marrow-derived mast cells (BMMCs) from C57BL/6 Wild type (WT) or KO (CD1dâ/â) mice were activated with Ag/Ab (refer to WT-act-BMMCs and KO-act-BMMCs, respectively) or α-Galactosylceramide (WT-αGal-BMMCs, KO-αGal-BMMCs) in the presence of iNKT cells. WT, KO or BMMC-transferred KO mice were sensitized and/or challenged by OVA or α-Gal to induce asthma. KO-act-BMMCs reduced intracellular Ca2 + levels, expression of signaling molecules (Ras, Rac1/2, PLA2, COX-2, NF-κB/AP-1), mediator release (histamines, leukotrienes and cytokines/chemokines), and total IgE levels versus the corresponding WT-BMMCs. KO mice reduced total and OVA-specific serum IgE levels, number of mast cells, recruiting molecules (CCR2/CCL2, VCAM-1, PECAM-1), expression of tryptase, c-kit, CD40L and cytokine mRNA, co-localization of c-kit and CD1d or iNKT cells in BAL cells or lung tissues, and PCA responses, compared with the corresponding WT mice. BMMC-transferred KO-both mice showed the restoration of all allergic responses versus KO-both mice (Ag/Ab reaction plus α-Gal). KO-αGal-BMMCs or KO-αGal mice did not show any responses. Our data suggest that CD1d-expressed mast cells may function as APC cells for iNKT cells and exacerbate airway inflammation and remodeling through up-regulating IgE production via B cell Ig class switching and mediator release in mast cells of OVA-challenged mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 26, Issue 5, May 2014, Pages 1105-1117
Journal: Cellular Signalling - Volume 26, Issue 5, May 2014, Pages 1105-1117
نویسندگان
Gwan Ui Hong, Nam Goo Kim, Tae Jin Kim, Jai Youl Ro,