Pertussis toxin-sensitive heterotrimeric Gαi/o proteins mediate WNT/β-catenin and WNT/ERK1/2 signaling in mouse primary microglia stimulated with purified WNT-3A

► We examined WNT-3A-induced β-catenin and ERK1/2 signaling in primary microglia. ► WNT-3A signaling to β-catenin and ERK1/2 requires heterotrimeric G proteins. ► PTX blocks WNT-3A-induced LRP6 and DVL phosphorylation and β-catenin stabilization. ► WNT-3A regulates ERK1/2 via βγ, PLC, Ca2 + and MEK1/2-dependent mechanisms. ► WNT-3A-induced COX2 expression is regulated via the WNT/ERK1/2 pathway.