کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10820431 | 1060632 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activity and function of rabbit muscle-specific creatine kinase at low temperature by mutation at gly268 to asn268
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Activity and function of rabbit muscle-specific creatine kinase at low temperature by mutation at gly268 to asn268 Activity and function of rabbit muscle-specific creatine kinase at low temperature by mutation at gly268 to asn268](/preview/png/10820431.png)
چکیده انگلیسی
Carp muscle-specific creatine kinase M1 isoenzyme (M1-CK) seems to have evolved to adapt to synchronized changes in body temperature and intracellular pH. When gly268 in rabbit muscle-specific creatine kinase was substituted with asn268 as found in carp M1-CK, the rabbit muscle-specific CK G286N mutant specific activity at pH 8.0 and 10 °C was more than 2-fold higher than that in the wild-type rabbit enzyme. Kinetic studies showed that Km values of the rabbit CK G268N mutant were similar to those of the wild-type rabbit enzyme, yet circular dichroism spectra showed that the overall secondary structures of the mutant enzyme, at pH 8.0 and 5 °C, were almost identical to the carp M1-CK enzyme. The X-ray diffraction pattern of the mutant enzyme crystal revealed that amino acid residues involved in substrate binding are closer to one another than in the rabbit enzyme, and the cysteine283 active site of the mutant enzyme points away from the ADP binding site. At pH 7.4-8.0 and 35-10 °C, with a smaller substrate, dADP, specific activities of the mutant enzyme were consistently higher than the wild-type rabbit enzyme and more similar to the carp M1-CK enzyme. Thus, the smaller active site of the RM-CK G268N mutant may be one of the reasons for its improved activity at low temperature.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 158, Issue 3, March 2011, Pages 189-198
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 158, Issue 3, March 2011, Pages 189-198
نویسندگان
Chih-Lu Wu, Ying-Hao Li, Hsi-Chieh Lin, Yi-Hang Yeh, Hong-Young Yan, Chwan-Deng Hsiao, Cho-Fat Hui, Jen-Leih Wu,