کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10820574 | 1060718 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Partial purification and characterization of pro-phospholipase A2 activating proteases from gill membranes of the red sea bream, Chrysophrys major
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کلمات کلیدی
PLA2PoPCSBTILBTILima Bean Trypsin Inhibitorp-APMSF1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine - 1-پالمیتویل-2-اولئویل-اسن-گلیسرو-3-فسفوشولینSecretory phospholipase A2 - اسید فسفولیپاز A2sodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدTrypsin-like protease - تریپسین مانند پروتئازPartial purification - تصفیه جزئیFish - ماهیsoy bean trypsin inhibitor - مهار کننده تریپسین لوبیا سویاGills - گیلز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We previously reported that gill group IB secretory phospholipase A2 (sPLA2) exists as an inactive pro-sPLA2 with the dipeptide Ala-Arg, at the N-terminus of mature sPLA2 in mucous cells. Pro-sPLA2 should be activated after being secreted to the surface of gill epithelia by trypsin-like protease. To clarify the above hypothesis, we investigated the existence of pro-sPLA2 activating protease (PAP) in the gills of the red sea bream, using gill pro-sPLA2 as a substrate. PAP was solubilized from the membrane fraction of the gills with 2% sodium cholate and partially purified by benzamidine-Sepharose chromatography and reversed-phase HPLC. Partially purified proteases, PAP1 and PAP2 showed a high molecular mass of about 200 kDa by gelatin zymography. PAP1 and PAP2 had optimal pH from 7 to 9 and were inhibited by trypsin inhibitors. These properties of PAP1 and PAP2 suggest that both enzymes belong to the membrane-associated trypsin-like serine protease family, such as enteropeptidase and corin. This is the first report verifying the existence of the activating protease of group IB pro-sPLA2 isoforms in a non-digestive tissue.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 141, Issue 1, May 2005, Pages 121-127
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 141, Issue 1, May 2005, Pages 121-127
نویسندگان
Satoshi Uchiyama, Noriaki Iijima,