کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10823509 | 1061873 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deficiency of the oxidative damage-specific DNA glycosylase NEIL1 leads to reduced germinal center B cell expansion
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کلمات کلیدی
LPSSHM8-oxoGActivation-induced cytidine deaminaseGerminal center B cellsformamidopyrimidines8-oxoguanine - 8-اکسوگوینینDNA glycosylase - DNA گلیکوزیلازOxidative damage - آسیب اکسیداتیوsomatic hypermutation - ابرمتن سوتیimmunoglobulin - ایمونوگلوبولینThymine glycol - تینین گلیکولlipopolysaccharide - لیپوپلی ساکاریدGerminal center - مرکز ژرمینالImmune response - پاسخ یا واکنش ایمنیAID - کمک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Mammalian cells possess multiple DNA glycosylases, including OGG1, NTH1, NEIL1, NEIL2 and NEIL3, for the repair of oxidative DNA damage. Among these, NEIL1 and NEIL2 are able to excise oxidized bases on single stranded or bubble-structured DNA and has been implicated in repair of oxidative damage associated with DNA replication or transcription. We found that Neil1 was highly constitutively expressed in the germinal center (GC) B cells, a rapidly dividing cell population that is undergoing immunoglobulin (Ig) gene hypermutation and isotype switching. While Neil1â/â mice exhibited normal B and T cell development and maturation, these mice contained a significantly lower frequency of GC B cells than did WT mice after immunization with a T-dependent antigen. Consistent with the reduced expansion of GC B cells, Neil1â/â mice had a decreased frequency of Ig gene hypermutation and produced less antibody against a T-dependent antigen during both primary and secondary immune responses. These results suggest that repair of endogenous oxidative DNA damage by NEIL1 is important for the rapid expansion of GC B cells and efficient induction of humoral immune responses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 8, Issue 11, 2 November 2009, Pages 1328-1332
Journal: DNA Repair - Volume 8, Issue 11, 2 November 2009, Pages 1328-1332
نویسندگان
Hiromi Mori, Rika Ouchida, Atsushi Hijikata, Hiroshi Kitamura, Osamu Ohara, Yingqian Li, Xiang Gao, Akira Yasui, R. Stephen Lloyd, Ji-Yang Wang,