کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10823645 | 1061914 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Histone acetylation, chromatin remodelling, transcription and nucleotide excision repair in S. cerevisiae: studies with two model genes
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کلمات کلیدی
LMPCRHDACsMNaseNTSS. cerevisiaeORFTCrcpd - CPDNER - DOWNUltraviolet - اشعه فرابنفشGGR - بارTranscription-coupled repair - تعمیر رونویسی همراهnucleotide excision repair - تعمیر مجدد نوکلئوتیدیGlobal genome repair - تعمیر ژنوم جهانیcyclobutane pyrimidine dimer - دییریر پیریمیدین cyclobutanetranscribed strand - رشته رونویسیnon-transcribed strand - رشته غیر رونویسیopen reading frame - قاب خواندن بازmicrococcal nuclease - نیکلاز میکروکوکالhistone deacetylases - هیستون deacetylaseshistone acetyltransferases - هیستون استیل ترانسفرازpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازChromatin - کروماتینHATs - کلاه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We describe the technology and two model systems in yeast designed to study nucleotide excision repair (NER) in relation to transcription and chromatin modifications. We employed the MFA2 and MET16 genes as models. How transcription-coupled (TCR) and global genome repair (GGR) operate at the transcriptionally active and/or repressed S. cerevisiae MFA2 locus, and how this relates to nucleosome positioning are considered. We discuss the role of the Gcn5p histone acetyltransferase, also associated with MFA2's transcriptional activation, in facilitating efficient NER at the transcriptionally active and inactive genes. The effect of Gcn5p's absence in reducing NER was local and UV stimulates Gcn5p-mediated histone acetylation at the repressed MFA2 promoter. After UV irradiation Swi2p is partly responsible for facilitating access to restriction of DNA in the cores of the nucleosomes at the MFA2 promoter. The data suggest similarities between chromatin remodelling for NER and transcription, yet differences must exist to ensure this gene remains repressed in α cells during NER. For MET16, we consider experiments examining chromatin structure, transcription and repair in wild type and cbf1Πcells under repressing or derepressing conditions. Cbf1p is a sequence specific DNA binding protein required for MET16 chromatin remodelling and transcription.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 4, Issue 8, 28 July 2005, Pages 870-883
Journal: DNA Repair - Volume 4, Issue 8, 28 July 2005, Pages 870-883
نویسندگان
Yumin Teng, Yachuan Yu, Jose A. Ferreiro, Raymond Waters,