کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10825350 | 1063599 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Soluble guanylate cyclase and neuronal nitric oxide synthase colocalize in rat nucleus tractus solitarii
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کلمات کلیدی
nNOSventral subnucleusSGCXIICOMDMVNTSPBSN-methyl-d-aspartateNMDAAMPANOSSDSConfocalalpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate - آلفا آمینو 3-هیدروکسی-5-متیل-4-ایزوکسول پروپیوناتγ-aminobutyric acid - اسید γ-آمینوبوتیریکImmunoreactivity - ایمنی فعالImmunofluorescent - ایمونوفلورسنتfourth ventricle - بطن چهارمsodium dodecyl sulphate - سدیم دودسیل سولفاتneuronal nitric oxide synthase - سنتاز اکسید نیتریک عصبیSoluble guanylate cyclase - سیکلاس گوانیلات حل کنندهarea postrema - طبقه آخرPhosphate buffered saline - فسفات بافر شورNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازhypoglossal nucleus - هسته hypoglossalNucleus tractus solitarii - هسته دستگاه انفرادیdorsal motor nucleus of vagus - هسته موتور پشتی وحشیGABA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Soluble guanylate cyclase and neuronal nitric oxide synthase colocalize in rat nucleus tractus solitarii Soluble guanylate cyclase and neuronal nitric oxide synthase colocalize in rat nucleus tractus solitarii](/preview/png/10825350.png)
چکیده انگلیسی
Nitric oxide has been implicated in transmission of cardiovascular signals in the nucleus tractus solitarii (NTS). Pharmacological studies suggest that activation of neurons by nitric oxide in the NTS may involve soluble guanylate cyclase (sGC). However, anatomical data supporting this suggestion have not been available. In this study, we tested the hypothesis that neurons and fibers containing neuronal nitric oxide synthase (nNOS) lie in close proximity to those containing sGC and the two enzymes colocalize in some neurons and fibers in the NTS. We perfused six rats and obtained brain stem sections for double immunofluorescent staining utilizing antibodies selective for sGC and for nNOS combined with confocal microscopy. The distribution and staining intensity of nNOS-immunoreactivity (IR) was similar to our earlier reports. IR of sGC was present in cell bodies, proximal dendrites and fibers of many brain stem regions. Strong sGC-IR was noted in the hypoglossal, dorsal motor nucleus of vagus and gracilis nuclei. The NTS exhibited moderate sGC-IR. Superimposed images showed that many NTS neurons contained both nNOS-IR and sGC-IR. The percentage of sGC-IR positive cells that were also nNOS-IR positive differed among NTS subnuclei. Similarly, the percentage of nNOS-IR positive cells that were also sGC positive differed among NTS subnuclei. Fibers stained for both nNOS-IR and sGC-IR were also present in NTS subnuclei. In addition, we identified fibers that were stained for nNOS-IR or sGC-IR alone and often found such singly labeled fibers apposed to each other. These data support our hypothesis and provide anatomical support for the suggestion that nitroxidergic activation of the NTS involves sGC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chemical Neuroanatomy - Volume 29, Issue 2, March 2005, Pages 127-136
Journal: Journal of Chemical Neuroanatomy - Volume 29, Issue 2, March 2005, Pages 127-136
نویسندگان
L.H. Lin, W.T. Talman,