کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10825844 | 1064679 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine
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کلمات کلیدی
dsDNAN-ethylmaleimideCtDNAG-quadruplexssDNACu2+ - Cu2 +Single-stranded DNA - DNA تک رشته ایcalf-thymus DNA - DNA تیروئید گوسالهdouble-stranded DNA - DNA رشته ایLabel-free - بدون برچسبcircular dichroism - رنگ تابی دورانیLuminescence - لومینسانس NEM - نهhistidine - هیستیدینIridium(III) complex - پیچیده ایرییدیم (III)
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A label-free G-quadruplex-based luminescent switch-on assay has been developed for the selective detection of micromolar histidine in aqueous solution. In this study, an iridium(III) complex was employed as a G-quadruplex-specific luminescent probe while a guanine-rich oligonucleotide (Pu27, 5â²-TG4AG3TG4AG3TG4A2G2-3â²)/cupric ion (Cu2+) ensemble was employed as a recognition unit for histidine. The initial luminescence of the iridium(III) complex in the presence of G-quadruplex DNA is effectively quenched by Cu2+ ions due to the Cu2+-mediated unfolding of the G-quadruplex motif. The addition of histidine sequesters Cu2+ ions from the ensemble, thereby restoring the luminescence of the system. The assay could detect down to 1 μM of histidine in aqueous media, and also exhibited good selectivity for histidine over other amino acids with the use of the cysteine, masking agent N-ethylmaleimide. Furthermore, the application of the assay for the detection of histidine in diluted urine samples was demonstrated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 64, Issue 3, 15 December 2013, Pages 205-211
Journal: Methods - Volume 64, Issue 3, 15 December 2013, Pages 205-211
نویسندگان
Hong-Zhang He, Modi Wang, Daniel Shiu-Hin Chan, Chung-Hang Leung, Jian-Wen Qiu, Dik-Lung Ma,