کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10826116 1064718 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of RNA-bound Tb3+ as a FRET donor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Use of RNA-bound Tb3+ as a FRET donor
چکیده انگلیسی
Lanthanide ions such as Tb3+ and Eu3+ have long been used to probe RNA and protein structures due to their luminescence properties and their steric and chemical similarities to biological metal ions such as Mg2+ and Ca2+. In this article, we introduce a method that utilizes the enhanced Tb3+ luminescence upon site-binding to RNA molecules as a FRET donor. Using this method, it is possible to identify specific metal ion-binding locations within a folded RNA molecule. Applications of this method include introducing FRET donors into molecules of interest by engineering RNA loops or bulges as Tb3+-binding pockets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 52, Issue 2, October 2010, Pages 173-179
نویسندگان
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