کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10840261 | 1067616 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Buckwheat (Fagopyrum esculentum Moench) low molecular weight seed proteins are restricted to the embryo and are not detectable in the endosperm
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کلمات کلیدی
IgEFagopyrum esculentumTEMEDtetramethylethylenediaminetris (hydroxymethyl) aminomethane hydrochlorideM.W.Endosperm - آندوسپرمsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدImmunoglobulin E - ایمونوگلوبولین ETris–HCl - تریس-HClMolecular weight - وزن مولکولیAllergenic proteins - پروتئین های آلرژیکCommon buckwheat - گندم سیاه معمولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Buckwheat (Fagopyrum esculentum Moench) low molecular weight seed proteins are restricted to the embryo and are not detectable in the endosperm Buckwheat (Fagopyrum esculentum Moench) low molecular weight seed proteins are restricted to the embryo and are not detectable in the endosperm](/preview/png/10840261.png)
چکیده انگلیسی
Buckwheat (Fagopyrum esculentum Moench) proteins are nutritionally important because of their high and balanced content of essential amino acids making their biological value much higher than that of cereal proteins. We analyzed extracts of buckwheat endosperm and embryo proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). On electropherograms of endosperm proteins, six intense bands were detected. Two most intense bands were in the range of molecular weights (M.W.s) from 50 to 60Â kDa. Protein of 57Â kDa has been shown not to cross-react against antibodies raised against proteins of M.W. ranging between 23 and 25Â kDa. There are no reports about the allergenicity of other endosperm proteins. On the electropherogram of buckwheat endosperm no low M.W. proteins could be detected. In this study we have demonstrated the tissue specific presence of proteins of different size classes of the endosperm and embryo tissues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 9, September 2005, Pages 862-865
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 9, September 2005, Pages 862-865
نویسندگان
Marija LiÄen, Ivan Kreft,