کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10840443 | 1067695 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of phosphate limitation on expression of genes involved in pyrimidine synthesis and salvaging in Arabidopsis
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کلمات کلیدی
UPRTaseUMPOMPFOACPSaseDHON-(phosphonacetyl)-l-aspartatePRPPDihydroorotateATCase5-Phosphoribosyl-1-pyrophosphate - 5-فسفریبوسیل-1-پری فسفاتArabidopsis - آرابیدوپسیس یا رشادیOrthophosphate - ارتوفسفات5-fluoroorotic acid - اسید 5-فلوروروتیکGene expression - بیان ژنPyrimidine nucleotide biosynthesis - بیوسنتز نوکلئوتید پیریمیدینAspartate transcarbamoylase - ترانس کرباموئیازاز آسپارتاتPhosphate - فسفاتPALA - پالاCarbamoyl phosphate synthetase - کارباموییل فسفات سنتتازPhosphate starvation - گرسنگی فسفات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Arabidopsis seedlings grown for 14Â d without phosphate (P) exhibited stunted growth and other visible symptoms associated with P deficiency. RNA contents in shoots decreased nearly 90%, relative to controls. In shoots, expression of Pht1;2, encoding an inducible high-affinity phosphate transporter, increased threefold, compared with controls, and served as a molecular marker for P limitation. Transcript levels for five enzymes (aspartate transcarbamoylase, ATCase, EC 2.1.3.2; carbamoyl phosphate synthetase, CPSase, EC 6.3.5.5); UMP synthase, EC 2.4.1.10, EC 4.1.1.23; uracil phosphoribosyltransferase, UPRTase, EC 2.4.2.9; UMP kinase, EC 2.7.1.14) increased 2-10-fold in response to P starvation in shoots. These enzymes, which utilize phosphorylated intermediates at putative regulated steps in de novo synthesis and salvaging pathways leading to UMP and pyrimidine nucleotide formation, appear to be coordinately regulated, at the level of gene expression. This response may facilitate pyrimidine nucleotide synthesis under P limitation in this plant. Expression of P-dependent and P-independent phosphoribosyl pyrophosphate (PRPP) synthases (PRS2 and PRS3, respectively) which provide PRPP, the phosphoribosyl donor in UMP synthesis via both de novo and salvaging pathways, was differentially regulated in response to P limitation. PRS2 mRNA levels increased twofold in roots and shoots of P-starved plants, while PRS3 was constitutively-expressed. PRS3 may play a novel role in providing PRPP to cellular metabolism under low P availability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 2, February 2005, Pages 91-99
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 2, February 2005, Pages 91-99
نویسندگان
Matthew M. Hewitt, Jessica M. Carr, Cynthia L. Williamson, Robert D. Slocum,