کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10840555 | 1067713 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gene silencing of cinnamyl alcohol dehydrogenase in Pinus radiata callus cultures
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کلمات کلیدی
Pinus radiataDHCADFRCRNAinterferenceNPT IIRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهenzyme linked immuno sorbent assay - آنزیم مرتبط با آزمون ایمونوژن جذب شده استELISA - تست الیزاCinnamyl alcohol dehydrogenase - سدیم هیدروژناز الکل سینامیلCAD - طراحی به کمک رایانه یا کَدtracheary element - عنصر ترسناکLignin - لیگنین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Gene silencing of cinnamyl alcohol dehydrogenase in Pinus radiata callus cultures Gene silencing of cinnamyl alcohol dehydrogenase in Pinus radiata callus cultures](/preview/png/10840555.png)
چکیده انگلیسی
Xylem-derived Pinus radiata cell cultures, which can be induced to differentiate tracheary elements (TEs), were transformed with an RNAi construct designed to silence cinnamyl alcohol dehydrogenase (CAD), an enzyme involved in the biosynthesis of monolignols. Quantitative enzymatic CAD measurements revealed reduced CAD activity levels in most transclones generated. TEs from transclones with ~20% residual CAD activity did not release elevated levels of vanillin, which was derived from coniferyl-aldehyde through a mild alkali treatment. However, the activation of the phenylpropanoid pathway in transclones with ~20% residual CAD activity through the application of non-physiological concentrations of sucrose and l-phenylalanine produced phenotypic changes. The accumulation of metabolites such as dihydroconiferyl-alcohol (DHCA), which also accumulates in the P. taeda CAD mutant cad-n1, was observed. These results indicate that a substantial reduction in CAD activity is necessary for this enzyme to become a rate-limiting step in lignin biosynthesis in conifers such as P. radiata and confirm that transformable P. radiata callus cultures can be useful to investigate the function of xylogenesis-related genes in conifers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 12, December 2005, Pages 1061-1066
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 12, December 2005, Pages 1061-1066
نویسندگان
Ralf Möller, Diane Steward, Lorelle Phillips, Heather Flint, Armin Wagner,