Differential gene expression related to an epigenetic memory affecting climatic adaptation in Norway spruce

Sequencing and annotation revealed considerable differences in the transcriptome of WE versus CE originated plants. By using qRT-PCR we studied the expression patterns of 32 selected candidate genes chosen from subtractive cDNA libraries analysis and nine siRNA pathways genes by a direct candidate approach. Eight genes, two transposons related genes, three with no match to Databases sequences and three genes from siRNA pathways (PaDCL1 and 2, PaSGS3) showed differential expression in progeny from CE and WE correlated with the family phenotypic differences. These findings may contribute to our understanding of the epigenetic mechanisms underlying adaptive changes acquired during embryogenesis.