کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10841016 | 1067877 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential change in root protein patterns of two wheat varieties under high and low nitrogen nutrition levels
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کلمات کلیدی
GDH2-DETriticum aestivum L.NO3two dimensional electrophoresis - الکتروفورز دو بعدیNitrogen nutrition - تغذیه نیتروژنDiversity - تنوعNitrate - نیتراتnitrate reductase - نیترات ردوکتازfresh weight - وزن ترdry weight - وزن خشکProteome - پروتئومglutamate dehydrogenase - گلوتامات دهیدروژنازGlutamine synthetase - گلوتامین سنتتاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Nitrogen deficiency tolerance is necessary to breed for wheat varieties adapted to low input management systems. The aim of this study was to identify root proteins differentially expressed in response to a nitrogen stress. Two wheat varieties were experimented at low (0.5 mM nitrate) and at high (3.0 mM nitrate) nitrogen levels. After 8 weeks, root samples were taken to assess enzyme activities and to perform two dimensional gel electrophoresis. A total of 860 root protein spots were qualitatively or quantitatively analysed. A significant variety effect existed for 74 spots, while a significant nitrate supply-dependent variation was observed for 56 spots. Moreover 20 spots showed a significant variety à N-supply effect. Correlations were made between physiological parameters (nitrogen content and enzyme activities) and the intensity of variable spots. Positive correlations were observed for nitrate reductase and glutamate dehydrogenase activities and the intensity of a number of root proteins. This approach may be a way to identify new structural or regulatory proteins involved in the response to the level of nitrogen fertilisation in different wheat varieties.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 168, Issue 1, January 2005, Pages 81-87
Journal: Plant Science - Volume 168, Issue 1, January 2005, Pages 81-87
نویسندگان
Nasser Bahrman, Aurélia Gouy, Florence Devienne-Barret, Bertrand Hirel, Françoise Vedele, Jacques Le Gouis,