کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10841376 | 1067891 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Coordinated responses of phytochelatins and metallothioneins to heavy metals in garlic seedlings
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کلمات کلیدی
Allium sativumγ-ECPhytochelatinMTSCysTFAGSHphytochelatinsγ-glutamylcysteine - γ-گلوتامیل سیستئینTrifluoroacetic acid - اسید TrifluoroaceticAOS - بهHeavy metal tolerance - تحمل فلزات سنگینTranscriptional regulation - تنظیم ترانزیتیPCs - رایانه های شخصیCysteine - سیستئینMetallothionein - متالوتونیئینMetallothioneins - متالوتیژیونCadmium - کادمیمhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراGlutathione - گلوتاتیونactive oxygen species - گونه های اکسیژن فعال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Coordinated responses of phytochelatins and metallothioneins to heavy metals in garlic seedlings Coordinated responses of phytochelatins and metallothioneins to heavy metals in garlic seedlings](/preview/png/10841376.png)
چکیده انگلیسی
To evaluate the roles of phytochelatins (PCs) and metallothioneins (MTs) in heavy metal tolerance in garlic (Allium sativum L.), the cDNAs encoding a phytochelatin synthase and a type 2 MT were cloned from the seedlings of garlic using RACE method and designated AsPCS1 and AsMT2a. Semi-quantitative reverse transcriptase-PCR and PCs contents showed transcript levels of AsPCS1 in roots exposed to cadmium increased significantly within 1Â h concomitant with a sharp increase of PCs. However, the RNA expression of AsMT2a in roots was slightly increased within the preliminary phases of cadmium treatment and an obvious increase did not occurred until 10Â h of Cd exposure. Different effects on RNA expression of AsPCS1 and AsMT2a appeared under varied stresses. Treatment with cadmium, arsenic and heat shock resulted in a strong increase of AsPCS1 transcripts and PCs contents in roots. Theses results showed that AsPCS1 and AsMT2a displayed mutual coordination under the stresses. The implications of these results with respect to differential regulation of AsPCS1 and AsMT2a during heavy metal exposure are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 169, Issue 6, December 2005, Pages 1059-1065
Journal: Plant Science - Volume 169, Issue 6, December 2005, Pages 1059-1065
نویسندگان
Haiyan Zhang, Wenzhong Xu, Jiangbo Guo, Zhenyan He, Mi Ma,